Analysis of Skin Microbiota in girls with vulvar lichen sclerosus

Study ObjectsVulvar lichen sclerosus (VLS) is a chronic skin disease that affects the of vulva with itching, irritation, and pain. It may present in prepubertal or adolescent patient. The etiology VLS remains unclear. Autoimmune, genetic, hormonal, infectious conditions be involved pathogenesis [1]. Recent studies suggested unbalanced microbiota region [2-4]. We thus hypothesized alterations microbiotas contribute to development VLS. To evaluate compositional characteristics differences vulvar microbiota, we performed pilot case-control study by comparing girls Labial adhesions healthy controls.Materials MethodsWe recruited total 45 patients outpatient Pediatric Adolescent Gynecology clinic Children's Hospital Zhejiang University from October 2021 June 2022. Vulvar dermal swab samples (n=19), Nevus without specific vulvovaginitis (n=11), (n=15) were obtained. DNA was extracted all samples. V4 fragments 16S rRNA amplified for high-throughput sequencing analyzed QIIME2 [5]. composition between other two groups α diversity, β LEfSe analysis (http://huttenhower.sph.harvard.edu/galaxy/).ResultsOur results showed average age onset 6.52 (Table 1). There no diversity (Kruskal-Wallis test; P=0.06 Faith_pd index, Figure 1a; P=0.58 Pielou 1b) among three groups. In terms principal coordinate (PCoA) statistical taxa these (PERMANOVA Jaccard, p=0.001, 1c; unweighted UniFrac, 1d). Our revealed had statistically higher relative abundance Parvimonas spp., Helcococcus Peptococcus spp. compared controls, Methylobacterium Moryella Lactobacillus adhesions.ConclusionParvimonas are specifically Cutaneous dysbiosis provide chance explain new concepts its treatment. controls. (http://huttenhower.sph.harvard.edu/galaxy/). adhesions.